Characterisation of an extracellular serine protease gene (naspgene) fromDermatophilus congolensis
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چکیده
منابع مشابه
Characterisation of an extracellular serine protease gene (nasp gene) from Dermatophilus congolensis.
A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp i...
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We have purified a minor extracellular serine protease from a strain of Bacillus subtilis bearing null mutations in five extracellular protease genes: apr, npr, epr, bpr, and mpr (A. Sloma, C. Rudolph, G. Rufo, Jr., B. Sullivan, K. Theriault, D. Ally, and J. Pero, J. Bacteriol. 172:1024-1029, 1990). During purification, this novel protease (Vpr) was found bound in a complex in the void volume a...
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Graduate Institute of Ag ricu It u ra I C he m i s t ry, National Taiwan University, Taipei, Taiwan 106, Republic of China The structural gene prtVp encoding the extracellular protease of Vibrio parahaemolyticus strain 93 was cloned in Escherichia coli and sequenced. The cloned DNA fragment contained a 1761 bp ORF encoding a 587 amino acid protein. The deduced polypeptide is composed of a 25 am...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2004
ISSN: 0378-1097,1574-6968
DOI: 10.1016/s0378-1097(03)00958-3